Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B
نویسندگان
چکیده
منابع مشابه
Residue-specific global fluorination of Candida antarctica lipase B in Pichia pastoris.
We report the in vivo fluorination of the tryptophan, tyrosine, and phenylalanine residues in a glycosylation-deficient mutant of Candida antarctica lipase B, CalB N74D, expressed in the methylotrophic yeast Pichia pastoris and subsequently segregated into the growth medium. To achieve this, a P. pastoris strain auxotrophic for all three aromatic amino acids was supplemented with 5-fluoro-L-try...
متن کاملComparison of Yarrowia lipolytica and Pichia pastoris cellular response to different agents of oxidative stress.
Yeast cells exposed to adverse conditions employ a number of defense mechanisms in order to respond effectively to the stress effects of reactive oxygen species. In this work, the cellular response of Yarrowia lipolytica and Pichia pastoris to the exposure to the ROS-inducing agents' paraquat, hydrogen peroxide, and increased air pressure was analyzed. Yeast cells at exponential phase were expo...
متن کاملThe production of Yarrowia lipolytica lipase powder by improved spray-drying method
Lipase is used in the production of foods, flavor enhancers, detergents, cosmetics and pharmaceuticals. A common impediment to the production of commercial enzymes is their low-stability aqueous solutions. In this study, the downstream process was investigated to obtain a stable spray-dried lipase powder of Yarrowia lipolytica. The enzyme solution samples were supplemented with different concen...
متن کاملIntermediate Production of Mono- and Diolein by an Immobilized Lipase from Candida antarctica
Lipase from Candida antarctica, fixed on macroporous acrylic resin, has been used for the intermediate production of mono- and diolein by hydrolysis of triolein. The effect of altering concentrations of triolein and glycerol and the function of the molecular sieve on the hydrolysis reaction of triolein were investigated. The highest hydrolysis yield was observed for the utmost concentration of ...
متن کاملRational redesign of Candida antarctica lipase B
This thesis describes the use of rational redesign to modify the properties of the enzyme Candida antarctica lipase B. Through carefully selected single-point mutations, we were able to introduce substrate-assisted catalysis and to alter the reaction specificity. Other single-point mutations afforded variants with greatly changed substrate selectivity and enantioselectivity. Mutation of the cat...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-58683-3